Next-Generation Sequencing Libraries
Next-Generation Sequencing Library Preparation
A fundamental challenge for next-generation sequencing (NGS) is to deliver the promise of cost-effective use of the massive sequence data being generated. A major impediment is the complexity of preparing sequencer-ready libraries. Generating the DNA libraries (paired-end DNA or mate-paired DNA) or RNA libraries (RNASeq) that are used to feed NGS sequencers is a tedious and time-consuming manual process. Preparing a small number of libraries can take an entire day or more—with extensive ‘hands-on’ time. This labor-intensive approach has prevented most researchers from preparing more than four libraries in parallel.
IntegenX™ has introduced the Apollo 324™, an automated DNA library preparation platform, to address the rapidly growing NGS library preparation bottleneck. The Apollo 324 automates the preparation of up to 32 libraries per day and significantly reduces the most laborious aspects of the process, requiring only about an hour of manual effort. IntegenX is the first company to offer an automated library preparation system that supports most—and soon all—common next-generation sequencers. If your facility has more than one NGS platform, the Apollo 324 can automate virtually all your library preparations.
IntegenX offers a selection of platform-specific DNA Library preparation kits. Each kit is easy to use for setting up and running the protocol.
The Future of NGS
Sample barcoding techniques to consolidate multiple samples per run and increase throughput are becoming more widespread, so the number of libraries that will be required in the future will be much greater—as many as 96 libraries per run of a NGS sequencer.
IntegenX is developing a fully automated platform to meet the future needs of NGS DNA library preparation. Like our Apollo 100XL instrument that integrates sample handling, low volume microfluidics (with our patented MOVe™ technology), and optimized IntegenX-Certified™ reagents, this new platform will enable researchers to process many samples in parallel, using smaller reagent volumes, resulting in better quality libraries produced in less time and at lower cost.