Whole exome sequencing has emerged as a viable alternative to whole genome sequencing for detecting DNA changes (SNPs and insertions/deletions) of biological significance. Existing manual library construction methods give variable results due to the number of steps involved. Additionally, most studies require the construction of libraries from many different subjects, which consumes many days of laboratory staff time. Therefore, automating library construction is highly desirable because is permits the generation of more consistent libraries using fewer resources and less hands-on time.
This application note describes a protocol derived from standard IntegenX Apollo 324™ PrepX™ DNA or PrepX 32i protocols, which was developed by scientists at the Cincinnati Children’s Hospital Medical Center. This protocol uses the NimbleGen SeqCap EZ Library SR protocol to create pooled, indexed exome libraries followed by sequencing on an Illumina platform.
Download Using the Apollo 324 System to Create Libraries for NimbleGen SeqCap EZ Library SR (4 pages, pdf)