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RapidHIT® System

The First Fully Automated Rapid DNA System. Now with NDIS Approved Chemistry!

The RapidHIT System is the first fully automated sample-to-answer system for STR-based human identification. Now samples processed using our GlobalFiler® Express cartridges can be uploaded to the Combined DNA Index System (CODIS).

The self-contained human identification system produces standardized DNA profiles from buccal swabs and other human samples in ~90 minutes. The RapidHIT System uses gold standard sample extraction, Polymerase Chain Reaction (PCR) and Capillary Electrophoresis (CE) technologies. Requiring only three minutes of hands-on time to generate one through seven DNA profiles, the easy to use system fits into any forensic workflow. The output from the system is compatible with local, state and national human identification databases.

  • >90%+ full concordant profiles for expanded core CODIS loci
  • 100% concordance with NIST SRM 2391c standard
  • Run 1 sample, 7 samples, or anything in between
  • Process any swab type or crime scene samples directly in the cartridge
  • Requires on ~3 minutes of hands-on time, less than 2 hours time to results

RapidHIT Downloads

The Case for Rapid DNA

Overview Brochure

Validation and Applications

GlobalFiler Brochure

RapidHIT® GlobalFiler® Express Kit

NDIS Approved GlobalFiler® Express Chemistry for CODIS Upload

  • >90% full profile and CODIS success rate
  • Run 1 sample, 7 samples, or anything in between
  • Process any swab type or crime scene samples directly in the cartridge
  • Requires only ~3 minutes of hands-on time, less than 2 hours time to results

High Sensitivity and Accuracy

  • Buccal Swabs: 100% concordance for DNA levels equivalent to one cheek touch up to 20 cheek swipes.
  • Blood: 100% concordance for DNA levels equivalent to as low as 1.0 μL of blood on both cotton swab and FTA paper.

Concordance evaluated using a series of cheek swipe reference samples from 2 donors,

Figure 1. Concordance evaluated using a series of cheek swipe reference samples from 2 donors, including 1 cheek touch, 1 swipe, 2 swipes, 5 swipes, 10 swipes and 20 swipes of the cheek (N = 3). A total of 36 swabs were tested. Full profile concordance was measured for all swabs.

Concordance evaluated using a series of blood reference samples of 20 μL, 10 μL, 5 μL, 2.5 μL and 1.0 μL on both cotton swab and FTA paper (N = 3). A total of 30 samples were tested. Full profile concordance was measured for all samples.
Figure 2. Concordance evaluated using a series of blood reference samples of 20 μL, 10 μL, 5 μL, 2.5 μL and 1.0 μL on both cotton swab and FTA paper (N = 3). A total of 30 samples were tested. Full profile concordance was measured for all samples.
 

High-Quality Investigative Leads in Under Two Hours

Investigative Leads with Saliva-Based Samples

Investigative Leads with Saliva-Based Samples
Figure 3. Concordance of saliva-based sample types—cigarette, soda can, water bottle, drinking straw, sunflower seed husk, and chewing gum (N = 3).

Victim Identification With Tissue Samples

Victim Identification With Tissue Samples
Figure 4. Electropherogram of full DNA profile generated from muscle tissue deposited onto a swab. Full profiles have also been obtained from other tissue types such as liver and lung.
 

Full Concordance In The Presence of Inhibitors

Table 1. Full concordant profiles on the RapidHIT® System in the presence of common inhibitors.
Inhibitor and Sample Range Tested with Full Concordant Profiles
Hematin on swab 0 –2 mM
Coffee on swab 0 – 100 µL
Chewing tobacco slurry on swab 0 – 100 µL
EDTA in STR premix 0 – 1 mM

GlobalFiler® Express Kit Specifications

 Specification Description
Samples Per Run 1 to 7 samples
Sample Types Buccal swabs, blood and casework samples
Number of Loci 24 Loci10 mini-STR Loci
Allelic Ladder GlobalFiler® Express Kit allelic ladder with 343 alleles
Hands-on Time ~3 minutes
Run time Less than 2 hours
Storage Refrigerated (4-10 °C) Sample and Buffer cartridges can be stored at room temperature up to 3 months.